Overview
Assay information
| Name: | ELISpot (IFN-gamma Enzyme-Linked ImmunoSpot) |
| Methodology: | ELISpot |
| Target area: | Adaptive: T-cell and Cellular |
Description
The IFN-gamma Enzyme-Linked ImmunoSpot (ELISpot) assay is used to detect the secretion of IFN-gamma by immune cells in response to a specific stimulus. Live cells are stimulated with peptide pools derived from the protein sequence under investigation. The IFN-gamma secreted by antigen-specific cells is captured and visualized using a colorimetric reaction, allowing for the enumeration of antigen-specific cells using an ELISpot counter.
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