Overview
Publication
AIDS Res Hum Retroviruses. 2017 Apr; 33(4):368-372.
PubMed ID: 27758116
Title
Short communication: Potential risk of replication-competent virus in HIV-1 Env-pseudotyped virus preparations
Authors
Bilska M, Tang H, Montefiori DC
Abstract
Env-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies. It is often assumed that all pseudovirus particles are capable of only a single round of infection, making them a safe alternative to work with live HIV-1. In this study, we show that some Env-pseudotyped virus preparations give rise to low levels of replication-competent virus. These levels did not compromise results in the TZM-bl neutralization assay; however, their presence highlights a need to adhere to the same level of biosafety when working with Env-pseudotyped viruses that are required for work with replication competent HIV-1.
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