Overview
Publication
J Immunol Methods. 2013 Nov 29; 397(43467):71-6.
PubMed ID: 24035708
Title
A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions
Authors
Boesch AW, Zhao Y, Landman AS, Garcia MR, Fahey JV, Wira CR, Ackerman ME
Abstract
Mucosal tissues represent the front line in defense against potential pathogens, and one means by which mucosa provide protection is via the secretion of antimicrobials which can interfere with potential pathogens as well as recruit and modify the responses of immune cells. Here we describe adaptation of ELISA assays to microsphere format, facilitating simultaneous quantification of antimicrobial peptides including elafin, MIP3α, HBD2, HBD3, SLPI, RANTES, SDF1, lactoferrin, LL-37, and HNP1-3. The multiplexed assay exhibits excellent reproducibility, shows linearity over a two order of magnitude concentration range for most analytes, is compatible with biological fluids such as cervicovaginal lavage fluid, and presents significant cost and sample savings relative to traditional ELISA assays.
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